Conjugation

Part:BBa_K2961003:Design

Designed by: Muhammad Nahin Khan   Group: iGEM19_CMUQ   (2019-10-16)


gRNA / crRNA fixed-loop repeat for Cpf1 / Cas12a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is usually left to the manufacturer of gRNAs to generate along with the target sequence for gRNA


Source

https://international.neb.com/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a


References